CD176 抗体
This anti-CD176 antibody is a 小鼠 单克隆 antibody detecting CD176 in IF 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
Quick Overview for CD176 抗体 (ABIN3025630)
抗原
CD176
(Thomsen Friedenreich Antigen (CD176))
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This CD176 antibody is un-conjugated
应用范围
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
A68-B-A11
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纯化方法
- PEG precipitation
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免疫原
- Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.
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亚型
- IgM kappa
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anti-Thomsen Friedenreich Antigen (CD176) antibody
CD176 适用: 人, 小鼠, 大鼠 IHC, IF, StM 宿主: 小鼠 Monoclonal A78-G-A7 unconjugated
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应用备注
- Optimal dilution of the Thomsen-Friedenreich Antigen antibody should be determined by the researcher.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Thomsen-Friedenreich Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD176 (Thomsen Friedenreich Antigen (CD176))
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别名
- Thomsen-Friedenreich Antigen
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背景
- Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
抗原
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