PLAP 抗体
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北京 101111
Quick Overview for PLAP 抗体 (ABIN3025398)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.
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亚型
- IgG2a kappa
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应用备注
- Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 1-2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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别名
- Placental Alkaline Phosphatase
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背景
- PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This mAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes.
抗原
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