p40 (p63 Delta) (AA 5-17) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in WB, FACS, IF 和 IHC (p). Suitable for 人, Cow, 小鼠 和 大鼠.
Quick Overview for p40 (p63 Delta) (AA 5-17) 抗体 (ABIN3025362)
抗原
p40 (p63 Delta)
适用
人, Cow, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- AA 5-17
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纯化方法
- Protein A affinity chromatography
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免疫原
- Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.
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亚型
- IgG
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p40 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues requires boiling sections in 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the p40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- p40 (p63 Delta)
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别名
- p40
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背景
- P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody's ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody probe as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
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基因ID
- 8626
抗原
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