c-MYC 抗体 (C-Term)
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Quick Overview for c-MYC 抗体 (C-Term) (ABIN3025307)
抗原
See all c-MYC (MYC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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无交叉反应
- 小鼠, 大鼠, 小鸡
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus was used as the immunogen for the Myc antibody.
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亚型
- IgG1 kappa
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应用备注
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Optimal dilution of the Myc antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
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别名
- Myc
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背景
- Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
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途径
- p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
抗原
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