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c-MYC 抗体 (C-Term)

This 小鼠 单克隆 antibody specifically detects c-MYC in WB, IF, FACS 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN3025307
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for c-MYC 抗体 (C-Term) (ABIN3025307)

抗原

See all c-MYC (MYC) 抗体
c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This c-MYC antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM237
  • 抗原表位

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    C-Term

    无交叉反应

    小鼠, 大鼠, 小鸡

    纯化方法

    Protein G affinity chromatography

    免疫原

    Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus was used as the immunogen for the Myc antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the Myc antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

    别名

    Myc

    背景

    Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

    途径

    p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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