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MLANA 抗体

The 小鼠 单克隆 anti-MLANA antibody (Clone SPM342) (ABIN3025225) specifically detects MLANA in IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN3025225
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for MLANA 抗体 (ABIN3025225)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MLANA antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

SPM342
  • 原理

    Anti-Melan-A Antibody (azide and preservative free)

    无交叉反应

    小鼠, 大鼠

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for this anti-Melan-A antibody.

    亚型

    IgG2b, lambda
  • 应用备注

    The optimal dilution of the anti-Melan-A antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    Melan-A

    背景

    Melan-A/MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

    UniProt

    Q16655
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