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MLANA 抗体

This 小鼠 单克隆 anti-MLANA antibody (Clone SPM342) specifically detects MLANA in IF, FACS 和 IHC (p). The antibody is reactive with 人 和 马 samples.
产品编号 ABIN3025225
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MLANA 抗体 (ABIN3025225)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

适用

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人, 马

宿主

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小鼠

克隆类型

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单克隆

标记

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This MLANA antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM342
  • 无交叉反应

    小鼠, 大鼠

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for this anti-Melan-A antibody.

    亚型

    IgG2b kappa
  • 应用备注

    The optimal dilution of the anti-Melan-A antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    Melan-A

    背景

    Melan-A/MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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