FTL 抗体 (AA 38-165)
Quick Overview for FTL 抗体 (AA 38-165) (ABIN3025210)
抗原
See all FTL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 38-165
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).
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亚型
- IgG2b
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Western blot: 1-2 μg/mL,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Ferritin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- FTL (Ferritin, Light Polypeptide (FTL))
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别名
- Ferritin (Light Chain Specific)
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背景
- Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
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基因ID
- 2512
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途径
- Transition Metal Ion Homeostasis
抗原
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