LHB 抗体
Quick Overview for LHB 抗体 (ABIN3025192)
抗原
See all LHB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant beta sub-unit of human LH was used as the immunogen for the Luteinizing Hormone beta antibody.
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亚型
- IgG1 kappa
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anti-Luteinizing Hormone beta Polypeptide (LHB) (AA 23-141) antibody
LHB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) (AA 21-141) antibodyLHB 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) (AA 21-141) antibodyLHB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) antibodyLHB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal LHb-1214 unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) antibodyLHB 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) (AA 21-141) antibodyLHB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) antibodyLHB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) (Internal Region) antibodyLHB 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Luteinizing Hormone beta Polypeptide (LHB) (AA 78-104), (C-Term) antibodyLHB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14582 unconjugated
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应用备注
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Optimal dilution of the Luteinizing Hormone beta antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min RT (1)
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Luteinizing Hormone beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- LHB (Luteinizing Hormone beta Polypeptide (LHB))
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别名
- Luteinizing Hormone beta
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物质类
- Hormone
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背景
- Luteinizing hormone (LH) is a glycoprotein. Each monomeric unit is a sugar-like protein molecule, two of these make the full, functional protein. Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor, however, the hCG beta subunit contains an additional 24 amino acids and the hormones differ in the composition of their sugar moieties. LH is synthesized and secreted by gonadotrophs in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle-stimulating hormone (FSH), it is necessary for proper reproductive function. In the female, an acute rise of LH levels triggers ovulation. In the male, where LH has also been called Interstitial Cell-Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a useful marker in classification of pituitary tumors and the study of pituitary disease.
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process
抗原
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