Biotin 抗体
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北京 101111
Quick Overview for Biotin 抗体 (ABIN3025157)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
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亚型
- IgG1
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anti-Biotin antibody
WB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ICC 宿主: 兔 Monoclonal BLR328M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ELISA, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibody适用: Various Species IHC 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody适用: Various Species WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyWB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.\. Confocal Microscopy,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,In situ Nucleic Acid Hybridization,Western: blot 1-3 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Biotin
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物质类
- Chemical
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背景
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
抗原
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