Cytokeratin 7 抗体
Quick Overview for Cytokeratin 7 抗体 (ABIN3025128)
抗原
See all Cytokeratin 7 (KRT7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human KRT7 protein (KRT7/760) and OTN 11 cells (OV-TL12/30) were used as the immunogen for the Cytokeratin 7 antibody cocktail.
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亚型
- IgG
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anti-Keratin 7 (KRT7) (AA 251-350) antibody
KRT7 适用: 人, 小鼠 WB, FACS, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 91-394) antibodyKRT7 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 394-457) antibodyKRT7 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 1-94) antibodyKRT7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 2-469) antibodyKRT7 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal rOV-TL12-30 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠, 小鼠 WB, IHC, IP 宿主: 小鼠 Monoclonal 12D7 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS, StM, ISt 宿主: 兔 Monoclonal KRT7-1499R unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT7-760-OV-TL12-30 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT7-903 unconjugated
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应用备注
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Optimal dilution of the Cytokeratin 7 antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cytokeratin 7 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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别名
- Cytokeratin 7
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背景
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
抗原
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