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NGFR 抗体

This anti-NGFR antibody is a 小鼠 单克隆 antibody detecting NGFR in WB, FACS, IF 和 IHC (p). Suitable for 人 和 Non-Human Primate.
产品编号 ABIN3025052
发货至: 中国

Quick Overview for NGFR 抗体 (ABIN3025052)

抗原

See all NGFR 抗体
NGFR (Nerve Growth Factor Receptor (NGFR))

适用

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人, Non-Human Primate

宿主

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小鼠

克隆类型

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单克隆

标记

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This NGFR antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

NGFR5-NTR-912
  • 无交叉反应

    小鼠, 大鼠

    产品特性

    It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

    纯化方法

    Protein G affinity chromatography

    免疫原

    NGFR from A875 melanoma cells (NGFR5) and recombinant human protein (NTR/912) were used as the immunogen for the NGF Receptor antibody cocktail.

    亚型

    IgG
  • 应用备注

    Optimal dilution of the NGF Receptor antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the NGF Receptor antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    NGFR (Nerve Growth Factor Receptor (NGFR))

    别名

    NGF Receptor

    背景

    It recognizes a glycoprotein of 75  kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

    途径

    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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