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Elastase 3B 抗体 (AA 82-238)

This anti-Elastase 3B antibody is a 小鼠 单克隆 antibody detecting Elastase 3B in ELISA, IF 和 IHC (p). Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN3024740
发货至: 中国
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Quick Overview for Elastase 3B 抗体 (AA 82-238) (ABIN3024740)

抗原

See all Elastase 3B (ELA3B) 抗体
Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

适用

  • 104
  • 21
  • 20
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 大鼠, 小鼠

宿主

  • 73
  • 40
小鼠

克隆类型

  • 85
  • 28
单克隆

标记

  • 42
  • 17
  • 8
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 1
  • 1
  • 1
  • 1
This Elastase 3B antibody is un-conjugated

应用范围

  • 60
  • 56
  • 54
  • 42
  • 36
  • 36
  • 20
  • 7
  • 7
  • 4
  • 4
  • 4
  • 3
ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

ELTS3B-2
  • 抗原表位

    • 81
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 82-238

    纯化方法

    Protein G

    免疫原

    A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

    亚型

    IgG1
  • 应用备注

    Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

    1. FFPE staining requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: 2-4 μg/mL, order BSA free format for coating,IF: 1-2 μg/mL, IHC-P: 1-2 μg/mL (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

    别名

    Elastase 3B

    背景

    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
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