CD99 抗体
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北京 101111
Quick Overview for CD99 抗体 (ABIN3024516)
抗原
See all CD99 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
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亚型
- IgM kappa
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anti-CD99 (CD99) (AA 1-185) antibody
CD99 适用: 人, 小鼠 FACS, IHC, WB, ELISA 宿主: 小鼠 Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 适用: 人 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 适用: 小鼠 FACS, IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) antibodyKD Validated CD99 适用: 人 FACS, WB 宿主: 兔 Monoclonal 23GB3395 unconjugated Recombinant Antibody
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE
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应用备注
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Optimal dilution of the CD99 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD99
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别名
- CD99
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背景
- CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]
抗原
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