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NGFR 抗体

This anti-NGFR antibody is a 小鼠 单克隆 antibody detecting NGFR in FACS, IF 和 IHC (p). Suitable for 人 和 大鼠.
产品编号 ABIN3024324
发货至: 中国

Quick Overview for NGFR 抗体 (ABIN3024324)

抗原

See all NGFR 抗体
NGFR (Nerve Growth Factor Receptor (NGFR))

适用

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人, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This NGFR antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

CDLA271
  • 无交叉反应

    小鼠, 大鼠

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the CD271 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the CD271 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the CD271 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    NGFR (Nerve Growth Factor Receptor (NGFR))

    别名

    CD271

    背景

    CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

    途径

    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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