CA 19-9 抗体
Quick Overview for CA 19-9 抗体 (ABIN3024244)
抗原
See all CA 19-9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Precipitin lines obtained after immuno-diffusion using mAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le) were used as the immunogen for the CA19-9 antibody.
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亚型
- IgM kappa
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anti-Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) antibody
CA 19-9 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 121SLE unconjugated
anti-Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) antibodyCA 19-9 适用: 人 ELISA, FACS, IHC (p), WB, IHC (fro) 宿主: 小鼠 Monoclonal 121SLE unconjugated
anti-Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) antibodyCA 19-9 适用: 人 IHC, StM 宿主: 兔 Monoclonal CA19-9-1390R unconjugated
anti-Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) antibodyCA 19-9 适用: 人 IHC, FACS, IF, ISt 宿主: 小鼠 Monoclonal SPM588 unconjugated
anti-Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) antibodyCA 19-9 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal 121SLE unconjugated
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应用备注
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Optimal dilution of the CA19-9 antibody should be determined by the researcher.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CA19-9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
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别名
- CA19-9
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背景
- CA19-9, a carbohydrate epitope expressed on a high MW (>400 kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.
抗原
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