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- 抗原 See all NEFH 抗体
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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适用
- 人, 大鼠, 小鼠, 小鸡, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NEFH antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
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This antibody reacts with a 200 kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.
Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.
- 克隆位点
- RT97
- 亚型
- IgG1
- Top Product
- Discover our top product NEFH Primary Antibody
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anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibodyNEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 豚鼠, 兔, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: Cow WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibodyNEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig WB, IHC, FACS, StM 宿主: 兔 Monoclonal NEFL-H-2324R unconjugated Recombinant Antibody
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Neurofilament Heavy antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- 别名
- Neurofilament Heavy (NEFH 产品)
- 别名
- NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
- 背景
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This antibody reacts with a 200 kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.
Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
- 基因ID
- 4744
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