pan Keratin 抗体 (PE)
Quick Overview for pan Keratin 抗体 (PE) (ABIN3023996)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯度
 - Protein G affinity chromatography
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免疫原
 - Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.
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亚型
 - IgG1
 
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应用备注
 - The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT (1)
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限制
 - 仅限研究用
 
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浓度
 - 0.1 mg/mL
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缓冲液
 - 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C
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储存方法
 - Store the pan Cytokeratin antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
 
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- pan Keratin (panKRT)
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别名
 - Pan Cytokeratin
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背景
 - Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
 
抗原
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