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c-MYC 抗体 (C-Term)

The 小鼠 单克隆 anti-c-MYC antibody (Clone MVH39-1) (ABIN3023974) specifically detects c-MYC in WB, IF 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN3023974
发货至: 中国
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Quick Overview for c-MYC 抗体 (C-Term) (ABIN3023974)

抗原

See all c-MYC (MYC) 抗体
c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This c-MYC antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

MVH39-1
  • 抗原表位

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    C-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    A C-terminal peptide was used as the immunogen for the c-Myc antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Titering of the c-Myc antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the c-Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

    别名

    C-Myc

    背景

    Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]

    途径

    p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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