HSPG2 抗体
Quick Overview for HSPG2 抗体 (ABIN3023902)
抗原
See all HSPG2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
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亚型
- IgG2a kappa
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anti-Heparan Sulfate Proteoglycan 2 (HSPG2) (AA 4149-4391) antibody
HSPG2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) antibodyHSPG2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) antibodyHSPG2 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) antibodyHSPG2 适用: 人, 小鼠, Cow, 鱼, 猴, Pig IHC, IF, FACS, ISt, StM 宿主: 大鼠 Monoclonal A7L6 unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) antibodyHSPG2 适用: 人, Cow WB, IHC, ELISA, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal A71 unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) antibodyHSPG2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) (AA 25-134) antibodyHSPG2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Heparan Sulfate Proteoglycan 2 (HSPG2) (AA 524-701) antibodyHSPG2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the Perlecan antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))
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别名
- Perlecan (HSPG)
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背景
- This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
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途径
- Glycosaminoglycan Metabolic Process, Lipid Metabolism
抗原
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