Nuclear Antigen 抗体
Quick Overview for Nuclear Antigen 抗体 (ABIN3023839)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
产品特性
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
-
亚型
- IgG1 kappa
-
-
anti-Nuclear Antigen antibody
适用: 人, 大鼠, 小鼠 IF, ICC, FACS, IHC 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, Non-Human Primate IF, ICC, FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
-
-
应用备注
-
Titering of the Nuclear Antigen antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- Nuclear Antigen
-
背景
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
抗原
-
