Keratin 10 抗体
Quick Overview for Keratin 10 抗体 (ABIN3023828)
抗原
See all Keratin 10 (KRT10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
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亚型
- IgG1 kappa
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anti-Keratin 10 (KRT10) (AA 153-456) antibody
KRT10 适用: 人 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼 IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) (AA 345-454) antibodyKRT10 适用: 人, 小鼠, 大鼠, 猴 IHC, WB, FACS, ELISA 宿主: 小鼠 Monoclonal 3C2F5 unconjugated
anti-Keratin 10 (KRT10) (AA 1-584) antibodyKRT10 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, Pig IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 1D8 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠 IHC, WB, StM 宿主: 小鼠 Monoclonal LH2 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VIK-10 unconjugated
anti-Keratin 10 (KRT10) (Internal Region) antibodyKRT10 适用: 人, 小鼠, 大鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-610M unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the Keratin 10 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Keratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Keratin 10 (KRT10)
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背景
- This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
抗原
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