Mitochondria 抗体
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北京 101111
Quick Overview for Mitochondria 抗体 (ABIN3023781)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 小鼠, 大鼠
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产品特性
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.
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亚型
- IgG1 kappa
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anti-Mitochondria antibody
适用: 人 IHC, WB, IF, ELISA, FM 宿主: 小鼠 Monoclonal 113-1 unconjugated
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应用备注
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Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Mitochondria
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背景
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
抗原
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