Complement Factor H 抗体 (AA 20-270)
(2 references)
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北京 101111
Quick Overview for Complement Factor H 抗体 (AA 20-270) (ABIN3023097)
抗原
See all Complement Factor H (CFH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 20-270
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序列
- DCNELPPRRN TEILTGSWSD QTYPEGTQAI YKCRPGYRSL GNVIMVCRKG EWVALNPLRK CQKRPCGHPG DTPFGTFTLT GGNVFEYGVK AVYTCNEGYQ LLGEINYREC DTDGWTNDIP ICEVVKCLPV TAPENGKIVS SAMEPDREYH FGQAVRFVCN SGYKIEGDEE MHCSDDGFWS KEKPKCVEIS CKSPDVINGS PISQKIIYKE NERFQYKCNM GYEYSERGDA VCTESGWRPL PSCEEKSCDN P
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交叉反应
- 人, 小鼠, 大鼠
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产品特性
- Polyclonal Antibodies
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纯化方法
- Affinity purification
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免疫原
- Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Complement factor H (NP_001014975.1).
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亚型
- IgG
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anti-Complement Factor H (CFH) (AA 915-1156) antibody
CFH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (Internal Region) antibodyVerified CFH 适用: 人 WB, IHC, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 860-1231) antibodyCFH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 975-1217) antibodyCFH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 751-780) antibodyCFH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB18698 unconjugated
anti-Complement Factor H (CFH) (N-Term) antibodyCFH 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 975-1217) antibodyCFH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Complement Factor H (CFH) (AA 20-270) antibodyCFH 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 1-449) antibodyCFH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor H (CFH) (AA 751-780), (Middle Region) antibodyCFH 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200
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限制
- 仅限研究用
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- by
- Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo
- No.
- #101403
- 日期
- 2017.07.19
- 抗原
- Commplement Factor H
- Lot Number
- 4390101
- Method validated
- Immunofluorescence
- Positive Control
- MRL/lpr lupus mice (14 weeks of age)
- Negative Control
- FH knockout MRL/lpr lupus mice
- Notes
- Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
- Primary Antibody
- ABIN3023097
- Secondary Antibody
- goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
- Full Protocol
- Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
- Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
- Sections were cut at 8µm using the cryostat.
- Dry sections for 30min at RT.
- Hydrate sections in PBS 2x for 15min at RT.
- Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
- Wash sections 2x with PBS for 15min at RT.
- Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
- Wash sections 2x for 15min with PBS.
- Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
- Experimental Notes
生效 #101403 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.]()
IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.
Full Methods -
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze / thaw cycles
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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: "Role of alpha-1 antitrypsin in Bruch's membrane integrity." in: Scientific reports, Vol. 15, Issue 1, pp. 12223, (2025) (PubMed).
: "HK2 Mediated Glycolytic Metabolism in Mouse Photoreceptors Is Not Required to Cause Late Stage Age-Related Macular Degeneration-Like Pathologies." in: Biomolecules, Vol. 11, Issue 6, (2021) (PubMed).
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: "Role of alpha-1 antitrypsin in Bruch's membrane integrity." in: Scientific reports, Vol. 15, Issue 1, pp. 12223, (2025) (PubMed).
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- Complement Factor H (CFH)
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别名
- CFH
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背景
- This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,CFH,AHUS1,AMBP1,ARMD4,ARMS1,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Immunology & Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,CFH
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分子量
- 51 kDa/139 kDa
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基因ID
- 3075
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UniProt
- P08603
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途径
- Complement System, Cellular Response to Molecule of Bacterial Origin
抗原
-
