RPA3 抗体
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北京 101111
Quick Overview for RPA3 抗体 (ABIN302303)
抗原
See all RPA3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody detects Replication Protein A3 (14 kDa) (RPA3).
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产品特性
- Synonyms: REPA3, RP-A p14, RFA3, RF-A protein 3, Replication protein A, Replication protein A 14 kDasubunit, Replication factor A protein 3
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纯化方法
- Protein G column
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免疫原
- Full-length recombinant human RPA-14 expressed in E. coli
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亚型
- IgG1
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anti-Replication Protein A3, 14kDa (RPA3) (AA 12-121) antibody
RPA3 适用: 人 ELISA, WB, IHC (p), RNAi 宿主: 小鼠 Monoclonal 1F4 unconjugated
anti-Replication Protein A3, 14kDa (RPA3) (AA 1-121) antibodyRPA3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A3, 14kDa (RPA3) antibodyRPA3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A3, 14kDa (RPA3) (AA 1-119) antibodyRPA3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A3, 14kDa (RPA3) (AA 1-119) antibodyRPA3 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- Phosphate buffered saline
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储存条件
- -20 °C
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储存方法
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Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- RPA3 (Replication Protein A3, 14kDa (RPA3))
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背景
- Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.Synonyms: REPA3, RF-A protein 3, RFA3, RP-A p14, Replication factor A protein 3, Replication protein A, Replication protein A 14 kDa subunit
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基因ID
- 6119
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UniProt
- P35244
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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