VCP 抗体
(2 references)Quick Overview for VCP 抗体 (ABIN302102)
抗原
See all VCP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-VCP Purified
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特异性
- The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
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交叉反应 （详细）
- Mouse, Human
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纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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亚型
- IgM
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anti-Valosin Containing Protein (VCP) (N-Term) antibody
VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (C-Term) antibodyKO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 125-371) antibodyVCP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 1-588) antibodyVCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated
anti-Valosin Containing Protein (VCP) (AA 647-806) antibodyVCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) antibodyVCP 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody
anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibodyVCP 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 732-760) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (C-Term) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Flow cytometry: Intraacrosomal staining, recommended dilution: 3-12 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).
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: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
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- VCP (Valosin Containing Protein (VCP))
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别名
- VCP
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背景
- Valosin containing protein,VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,TERA, CDC48
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基因ID
- 7415
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UniProt
- P55072
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途径
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
抗原
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