IRF3 抗体 (AA 206-427)
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北京 101111
Quick Overview for IRF3 抗体 (AA 206-427) (ABIN297943)
抗原
See all IRF3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 206-427
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特异性
- Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60 % cross-reactivity with rmIRF3.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhIRF3 (aa206-427).
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亚型
- IgG
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anti-Interferon Regulatory Factor 3 (IRF3) (pSer396) antibody
IRF3 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-260) antibodyIRF3 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-452) antibodyIRF3 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3C8 unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (AA 51-150) antibodyIRF3 适用: 人, 小鼠, 大鼠, 猴, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-360) antibodyIRF3 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (pSer386) antibodyIRF3 适用: 人, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (C-Term) antibodyIRF3 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) antibodyIRF3 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4C3 unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) antibodyIRF3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-452) antibodyIRF3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 μg/mL (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 μg/mL detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4 % paraformaldehyde for 20 min at RT and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1 % BSA. ELISA: 0.5-1.0 μg/mL (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- IRF3 (Interferon Regulatory Factor 3 (IRF3))
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别名
- IRF3 / IRF-3
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背景
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Name/Gene ID: IRF3
Synonyms: IRF3, Interferon regulatory factor 3, IRF-3 -
基因ID
- 3661
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UniProt
- Q14653
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
抗原
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