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IRF3 抗体 (AA 206-427)

This anti-IRF3 antibody is a 山羊 多克隆 antibody detecting IRF3 in WB, ELISA, ICC 和 FACS. Suitable for 人.
产品编号 ABIN297943
发货至: 中国

Quick Overview for IRF3 抗体 (AA 206-427) (ABIN297943)

抗原

See all IRF3 抗体
IRF3 (Interferon Regulatory Factor 3 (IRF3))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This IRF3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
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    AA 206-427

    特异性

    Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60 % cross-reactivity with rmIRF3.

    纯化方法

    Affinity purified

    免疫原

    E. coli-derived rhIRF3 (aa206-427).

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

    Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 μg/mL (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 μg/mL detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4 % paraformaldehyde for 20 min at RT and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1 % BSA. ELISA: 0.5-1.0 μg/mL (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    40-50 % glycerol, PBS.

    浓度

    Lot specific

    缓冲液

    Lyophilized.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    IRF3 (Interferon Regulatory Factor 3 (IRF3))

    别名

    IRF3 / IRF-3

    背景

    Name/Gene ID: IRF3

    Synonyms: IRF3, Interferon regulatory factor 3, IRF-3

    基因ID

    3661

    UniProt

    Q14653

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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