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CD99 抗体 (Extracellular Domain)

This anti-CD99 antibody is a 山羊 多克隆 antibody detecting CD99 in FACS, WB 和 ELISA. Suitable for 人.
产品编号 ABIN296822
发货至: 中国

Quick Overview for CD99 抗体 (Extracellular Domain) (ABIN296822)

抗原

See all CD99 抗体
CD99

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This CD99 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), ELISA
  • 抗原表位

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    Extracellular Domain

    特异性

    Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.

    纯化方法

    Affinity purified

    免疫原

    NS0-derived rhCD99 extracellular domain.

    亚型

    IgG
  • 应用备注

    Approved: ELISA, Flo, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in 500 μL of Sterile PBS.

    浓度

    Lot specific

    缓冲液

    Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CD99

    别名

    CD99

    背景

    Name/Gene ID: CD99

    Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71

    基因ID

    4267
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