CD99 抗体 (Extracellular Domain)
Quick Overview for CD99 抗体 (Extracellular Domain) (ABIN296822)
抗原
See all CD99 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.
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纯化方法
- Affinity purified
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免疫原
- NS0-derived rhCD99 extracellular domain.
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亚型
- IgG
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应用备注
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Approved: ELISA, Flo, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 μL of Sterile PBS.
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浓度
- Lot specific
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缓冲液
- Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- CD99
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别名
- CD99
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背景
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Name/Gene ID: CD99
Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71 -
基因ID
- 4267
抗原
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