CD99 抗体 (Extracellular Domain)
-
- 抗原 See all CD99 抗体
- CD99
-
抗原表位
- Extracellular Domain
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This CD99 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), ELISA
- 特异性
- Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.
- 纯化方法
- Affinity purified
- 免疫原
- NS0-derived rhCD99 extracellular domain.
- 亚型
- IgG
- Top Product
- Discover our top product CD99 Primary Antibody
-
-
- 应用备注
-
Approved: ELISA, Flo, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute in 500 μL of Sterile PBS.
- 浓度
- Lot specific
- 缓冲液
- Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
-
- 抗原
- CD99
- 别名
- CD99 (CD99 产品)
- 物质类
- Viral Protein
- 背景
-
Name/Gene ID: CD99
Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71 - 基因ID
- 4267
-