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PKC beta 抗体 (AA 607-671)

PRKCB 适用: 人, 大鼠 WB, ELISA, ICC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN296089
发货至: 中国
  • 抗原 See all PKC beta (PRKCB) 抗体
    PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
    抗原表位
    • 16
    • 16
    • 14
    • 13
    • 11
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 607-671
    适用
    • 115
    • 94
    • 90
    • 14
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 135
    • 4
    • 2
    • 2
    • 1
    山羊
    克隆类型
    • 132
    • 12
    多克隆
    标记
    • 58
    • 14
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This PKC beta antibody is un-conjugated
    应用范围
    • 111
    • 60
    • 47
    • 29
    • 27
    • 26
    • 16
    • 12
    • 7
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    特异性
    Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
    纯化方法
    Affinity purified
    免疫原
    E. coli-derived rhPKCb1 (aa607-671).
    亚型
    IgG
    Top Product
    Discover our top product PRKCB Primary Antibody
  • 应用备注
    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    deionized water
    浓度
    Lot specific
    缓冲液
    Lyophilized.
  • 抗原
    PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
    别名
    PRKCB / PKC-Beta (PRKCB 产品)
    别名
    PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC-B antibody, Prkcb antibody, prkcb1 antibody, zgc:63591 antibody, PKC antibody, PRKCB_tv2 antibody, Pkc53E antibody, prkcb1l antibody, zgc:64063 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, protein kinase C, beta b antibody, protein kinase C, beta a antibody, PRKCB antibody, Prkcb antibody, prkcbb antibody, prkcba antibody
    背景
    Name/Gene ID: PRKCB
    Subfamily: PKC
    Family: Protein Kinase

    Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II
    基因ID
    5579
    UniProt
    P05771
    途径
    WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
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