PKC beta 抗体 (AA 607-671)
Quick Overview for PKC beta 抗体 (AA 607-671) (ABIN296089)
抗原
See all PKC beta (PRKCB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 607-671
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特异性
- Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhPKCb1 (aa607-671).
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亚型
- IgG
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anti-Protein Kinase C, beta (PRKCB) (AA 1-270) antibody
PRKCB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 220-340) antibodyPRKCB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (pThr500) antibodyPRKCB 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 517-643) antibodyPRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (pThr641) antibodyPRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (Internal Region) antibodyVerified PRKCB 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 642-673) antibodyPRKCB 适用: 人, 大鼠, 小鼠, 灵长类 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 601-673) antibodyPRKCB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 261-341) antibodyPRKCB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase C, beta (PRKCB) (AA 1-671) antibodyPRKCB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
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别名
- PRKCB / PKC-Beta
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背景
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Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II -
基因ID
- 5579
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UniProt
- P05771
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途径
- WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
抗原
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