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SMAD6 抗体 (AA 85-99)

The 兔 多克隆 anti-SMAD6 antibody is suitable to detect SMAD6 in samples from 人 和 Pig. It has been validated for WB, IHC 和 IHC (p).
产品编号 ABIN295868
发货至: 中国
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Quick Overview for SMAD6 抗体 (AA 85-99) (ABIN295868)

抗原

See all SMAD6 抗体
SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

适用

  • 45
  • 22
  • 10
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
人, Pig

宿主

  • 43
  • 14

克隆类型

  • 45
  • 12
多克隆

标记

  • 40
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SMAD6 antibody is un-conjugated

应用范围

  • 48
  • 29
  • 16
  • 11
  • 6
  • 5
  • 5
  • 3
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 10
    • 9
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 85-99

    特异性

    A 52 kD band is observed.

    预测反应

    Percent identity by BLAST analysis: Human, Pig (100%) Dog, Bovine, Opossum (93%) Gibbon, Marmoset, Rat (87%) Elephant (80%).

    纯化方法

    Protein G purified

    免疫原

    This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%), Dog, Bovine, Opossum (93%), Gibbon, Marmoset, Rat (87%), Elephant (80%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % BSA, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

    别名

    SMAD6

    背景

    Name/Gene ID: SMAD6

    Synonyms: SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6

    基因ID

    4091

    UniProt

    O43541

    途径

    Chromatin Binding
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