SMAD6 抗体 (AA 85-99)
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北京 101111
Quick Overview for SMAD6 抗体 (AA 85-99) (ABIN295868)
抗原
See all SMAD6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 85-99
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特异性
- A 52 kD band is observed.
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预测反应
- Percent identity by BLAST analysis: Human, Pig (100%) Dog, Bovine, Opossum (93%) Gibbon, Marmoset, Rat (87%) Elephant (80%).
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纯化方法
- Protein G purified
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免疫原
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This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%), Dog, Bovine, Opossum (93%), Gibbon, Marmoset, Rat (87%), Elephant (80%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.05 % BSA, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))
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别名
- SMAD6
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背景
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Name/Gene ID: SMAD6
Synonyms: SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6 -
基因ID
- 4091
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UniProt
- O43541
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途径
- Chromatin Binding
抗原
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