DLL1 抗体 (Extracellular Domain)
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Quick Overview for DLL1 抗体 (Extracellular Domain) (ABIN295726)
抗原
See all DLL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognize rat DLL1. Species sequence homology: mouse (97 % ), human (91 %), over aa18-537.
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纯化方法
- Affinity purified
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过滤
- 0.2 μm filtered
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免疫原
- NS0-derived rrDLL1 extracellular domain.
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亚型
- IgG
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anti-delta-Like 1 (DLL1) (Internal Region) antibody
Verified DLL1 适用: 人, 大鼠 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) (N-Term) antibodyDLL1 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) (AA 573-723) antibodyDLL1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) (AA 18-109) antibodyDLL1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4F8 unconjugated
anti-delta-Like 1 (DLL1) (N-Term) antibodyDLL1 适用: 人, 小鼠, 大鼠, Cow, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) (AA 150-175) antibodyDLL1 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) (AA 640-700) antibodyDLL1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) antibodyDLL1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 1 (DLL1) antibody (PE)DLL1 适用: 人, 小鼠, 大鼠 FACS 宿主: 仓鼠 Monoclonal HMD1-5 PE
anti-delta-Like 1 (DLL1) (AA 1-723) antibodyDLL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 μg/mL Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL Detection limit: 0.3 ng/well.~10 % cross-reactivity with rhDLL-1 and >1 % cross reactivity with rrNotch-1. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- DLL1 (delta-Like 1 (DLL1))
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别名
- DLL1
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背景
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Name/Gene ID: DLL1
Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa -
基因ID
- 28514
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UniProt
- O00548
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途径
- Notch Signaling, Stem Cell Maintenance
抗原
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