MEK1 + MEK2 抗体 (pSer217, Ser221)
MEK1 + MEK2
适用: 人, 小鼠, 大鼠
WB, IP
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all MEK1 + MEK2 products
- MEK1 + MEK2
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抗原表位
- pSer217, Ser221
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MEK1 + MEK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species cross-reactivity: Human, mouse and rat.
- 纯化方法
- Protein A purified
- 免疫原
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Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.
Type of Immunogen: Synthetic phospho-peptide - 亚型
- IgG
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anti-MEK1 + MEK2 (MEK1 + MEK2) (AA 189-238) antibody
MEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IP (1:50), WB (1:1000)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
- 储存液
- Without preservative
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- MEK1 + MEK2
- 别名
- MEK1 + MEK2 (MEK1 + MEK2 产品)
- 背景
- This antibody detects: MEK1 + MEK2
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