MEK1 + MEK2 抗体 (pSer217, Ser221)
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Quick Overview for MEK1 + MEK2 抗体 (pSer217, Ser221) (ABIN294413)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer217, Ser221
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特异性
- Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species cross-reactivity: Human, mouse and rat.
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纯化方法
- Protein A purified
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免疫原
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Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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anti-MEK1 + MEK2 (MEK1 + MEK2) (pSer221) antibody
MEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (pSer217) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (AA 193-242) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (AA 189-238) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IP (1:50), WB (1:1000)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MEK1 + MEK2
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别名
- MEK1 + MEK2
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背景
- This antibody detects: MEK1 + MEK2
抗原
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