SMAD2 抗体 (AA 234-249)
Our Local Distributor
北京 101111
Quick Overview for SMAD2 抗体 (AA 234-249) (ABIN293877)
抗原
See all SMAD2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 234-249
-
特异性
- The SMAD2 amino acid sequence used as immunogen is 100 % homologous in human (isoform 1:467 amino acids, isoform 2:437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
-
预测反应
- Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%) Panda, Stickleback, Pufferfish, Zebrafish (94%).
-
纯化方法
- Protein G purified
-
免疫原
- Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 were used as immunogen. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%), Panda, Stickleback, Pufferfish, Zebrafish (94%).
-
-
-
-
应用备注
-
Approved: IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, 0.05 % BSA, 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- avoid freeze thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
-
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
-
别名
- SMAD2
-
背景
-
Name/Gene ID: SMAD2
Synonyms: SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2 -
基因ID
- 4087
-
UniProt
- Q15796
-
途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
抗原
-