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SMAD2 抗体 (AA 234-249)

The 兔 多克隆 anti-SMAD2 antibody (ABIN293877) specifically detects SMAD2 in WB, IHC 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 猴, 犬, 马, Bat, Hamster 和 非洲爪蟾 samples.
产品编号 ABIN293877
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SMAD2 抗体 (AA 234-249) (ABIN293877)

抗原

See all SMAD2 抗体
SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

适用

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人, 小鼠, 大鼠, Cow, 兔, 小鸡, 猴, 犬, 马, Bat, Hamster, 非洲爪蟾

宿主

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克隆类型

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多克隆

标记

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This SMAD2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 234-249

    特异性

    The SMAD2 amino acid sequence used as immunogen is 100 % homologous in human (isoform 1:467 amino acids, isoform 2:437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.

    预测反应

    Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%) Panda, Stickleback, Pufferfish, Zebrafish (94%).

    纯化方法

    Protein G purified

    免疫原

    Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 were used as immunogen. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%), Panda, Stickleback, Pufferfish, Zebrafish (94%).
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % BSA, 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

    别名

    SMAD2

    背景

    Name/Gene ID: SMAD2

    Synonyms: SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2

    基因ID

    4087

    UniProt

    Q15796

    途径

    Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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