Renin 抗体 (AA 24-406)
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- 抗原 See all Renin (REN) 抗体
- Renin (REN)
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抗原表位
- AA 24-406
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适用
- 人
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Renin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Neutralization (Neut)
- 特异性
- Recognizes both the pro and mature forms of human Renin. In direct ELISAs and Western Blots, this antibody shows ~20 % cross-reactivity with rmRenin-1. Species sequence homology: mouse 70 %, canine 72 %.
- 纯化方法
- Affinity purified
- 免疫原
- NS0-derived rhRenin (aa24-406).
- 亚型
- IgG
- Top Product
- Discover our top product REN Primary Antibody
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anti-Renin (REN) (AA 67-153) antibody
REN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 24-406) antibodyREN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H2 unconjugated
anti-Renin (REN) (AA 275-389) antibodyREN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 6# unconjugated
anti-Renin (REN) (Internal Region) antibodyREN 适用: 人, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 331-406) antibodyREN 适用: 大鼠, 小鼠, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 67-153) antibodyREN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Renin (REN) (AA 65-264) antibodyREN 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 229-398) antibodyREN 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) antibodyREN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 24-406) antibodyREN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B5 unconjugated
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), IHC (3 - 10 μg/mL), IP, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Neutralization. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 3-10 μg/mL. Immunoprecipitation: Used to immunoprecipitate rhRenin from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhRenin. The antibody was preincubated with the activated enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 40 μg/mL (IC50) under conditions in which the enzyme was present at 2 μg/mL and the substrate concentration was 5uM. Considering the molecular masses of the enzyme (44kD) and the antibody (150 kD), IC50 was achieved at approximately 6:1 Molar ratio of the antibody to the enzyme. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 3 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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- 抗原
- Renin (REN)
- 别名
- REN / Renin 1 (REN 产品)
- 别名
- HNFJ2 antibody, RATRENAA antibody, RENAA antibody, Ren1 antibody, D19352 antibody, Ren antibody, Ren-1 antibody, Ren-A antibody, Ren1c antibody, Ren1d antibody, Rn-1 antibody, Rnr antibody, renin antibody, renin 1 structural antibody, REN antibody, Ren antibody, Ren1 antibody, ren antibody
- 背景
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Name/Gene ID: REN
Subfamily: Aspartic A1
Family: Protease
Synonyms: REN, Angiotensin-forming enzyme, Angiotensinogenase, HNFJ2, RENAA, Renin precursor, renal, Renin - 基因ID
- 5972
- UniProt
- P00797
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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