Renin 抗体 (AA 24-406)
Quick Overview for Renin 抗体 (AA 24-406) (ABIN293077)
抗原
See all Renin (REN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 24-406
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特异性
- Recognizes both the pro and mature forms of human Renin. In direct ELISAs and Western Blots, this antibody shows ~20 % cross-reactivity with rmRenin-1. Species sequence homology: mouse 70 %, canine 72 %.
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纯化方法
- Affinity purified
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免疫原
- NS0-derived rhRenin (aa24-406).
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亚型
- IgG
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anti-Renin (REN) (AA 67-153) antibody
REN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 24-406) antibodyREN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H2 unconjugated
anti-Renin (REN) (Internal Region) antibodyREN 适用: 人, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 331-406) antibodyREN 适用: 大鼠, 小鼠, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 67-153) antibodyREN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Renin (REN) (AA 275-389) antibodyREN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Renin (REN) (AA 65-264) antibodyREN 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 229-398) antibodyREN 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) antibodyREN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Renin (REN) (AA 24-406) antibodyREN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B5 unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), IHC (3 - 10 μg/mL), IP, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Neutralization. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 3-10 μg/mL. Immunoprecipitation: Used to immunoprecipitate rhRenin from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhRenin. The antibody was preincubated with the activated enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 40 μg/mL (IC50) under conditions in which the enzyme was present at 2 μg/mL and the substrate concentration was 5uM. Considering the molecular masses of the enzyme (44kD) and the antibody (150 kD), IC50 was achieved at approximately 6:1 Molar ratio of the antibody to the enzyme. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 3 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- Renin (REN)
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别名
- REN / Renin 1
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背景
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Name/Gene ID: REN
Subfamily: Aspartic A1
Family: Protease
Synonyms: REN, Angiotensin-forming enzyme, Angiotensinogenase, HNFJ2, RENAA, Renin precursor, renal, Renin -
基因ID
- 5972
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UniProt
- P00797
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原
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