Kallikrein 13 抗体 (AA 19-262)
Quick Overview for Kallikrein 13 抗体 (AA 19-262) (ABIN292875)
抗原
See all Kallikrein 13 (KLK13) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 19-262
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特异性
- Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10 % cross-reactivity with rhKallikrein 8, and less than 5 % cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.
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纯化方法
- Affinity purified
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免疫原
- NS0-derived rhKLK13 (aa19-262).
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亚型
- IgG
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anti-Kallikrein-Related Peptidase 13 (KLK13) (AA 25-261) antibody
KLK13 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kallikrein-Related Peptidase 13 (KLK13) (AA 151-250) antibodyKLK13 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Kallikrein-Related Peptidase 13 (KLK13) (AA 179-277) antibodyKLK13 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G9 unconjugated
anti-Kallikrein-Related Peptidase 13 (KLK13) (AA 71-120) antibodyKLK13 适用: 人, 小鼠, 犬, Cow, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IP, Neut, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 8.6 μg/mL (IC50) under conditions in which the enzyme was present at 1 μg/mL and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 Molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))
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别名
- KLK13 / Kallikrein 13
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背景
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Name/Gene ID: KLK13
Subfamily: Serine S1
Family: Protease
Synonyms: KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4 -
基因ID
- 26085
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UniProt
- Q9UKR3
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途径
- Complement System
抗原
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