GLI3 抗体
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- 抗原 See all GLI3 抗体
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GLI3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- 特异性
- Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived rhGLI-3.
- 亚型
- IgG
- Top Product
- Discover our top product GLI3 Primary Antibody
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anti-GLI Family Zinc Finger 3 (GLI3) antibody
GLI3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 41-57) antibodyGLI3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) antibodyGLI3 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) antibodyGLI3 适用: 人, 小鼠, 大鼠, 犬, 小鸡, 猴, Quail, Chimpanzee, Amphibian WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 800-900) antibodyGLI3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 40-57), (N-Term) antibodyGLI3 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 11-60) antibodyGLI3 适用: 人, 小鼠, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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- 抗原
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- 别名
- GLI3 (GLI3 产品)
- 别名
- ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody
- 背景
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Name/Gene ID: GLI3
Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3 - 基因ID
- 2737
- UniProt
- P10071
- 途径
- Hedgehog Signaling
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