GLI3 抗体
Quick Overview for GLI3 抗体 (ABIN292775)
抗原
See all GLI3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhGLI-3.
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亚型
- IgG
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anti-GLI Family Zinc Finger 3 (GLI3) antibody
GLI3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 303-561) antibodyGLI3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 30-60) antibodyGLI3 适用: 人 WB, IHC, ELISA, IF, FM, MA 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 111-343) antibodyGLI3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 800-900) antibodyGLI3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 40-57), (N-Term) antibodyGLI3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 11-60) antibodyGLI3 适用: 人, 小鼠, 大鼠 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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别名
- GLI3
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背景
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Name/Gene ID: GLI3
Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3 -
基因ID
- 2737
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UniProt
- P10071
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途径
- Hedgehog Signaling
抗原
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