NOTCH4 抗体 (AA 1778-2003)
Quick Overview for NOTCH4 抗体 (AA 1778-2003) (ABIN291848)
抗原
See all NOTCH4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1778-2003
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特异性
- Recognizes human Notch4.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhNotch-4 (aa1778-2003).
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亚型
- IgG
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anti-Notch 4 (NOTCH4) (AA 1715-2001) antibody
NOTCH4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (Middle Region) antibodyNOTCH4 适用: 人, 大鼠, Cow, 马, 豚鼠, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) antibodyNOTCH4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (AA 371-420) antibodyNOTCH4 适用: 人, Cow, 马, 豚鼠, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (AA 1261-1273) antibodyVerified NOTCH4 适用: 人, 小鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (AA 1824-2003) antibodyNOTCH4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (Middle Region) antibodyNOTCH4 适用: 人, Cow, 马, 豚鼠, Pig, 犬, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (Cleaved-Val1432) antibodyNOTCH4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (C-Term) antibodyNOTCH4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Notch 4 (NOTCH4) (AA 1819-1851), (C-Term) antibodyNOTCH4 适用: 人 WB 宿主: 兔 Polyclonal RB52895 unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- NOTCH4 (Notch 4 (NOTCH4))
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别名
- NOTCH4
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背景
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Name/Gene ID: NOTCH4
Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4 -
基因ID
- 4855
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UniProt
- Q99466
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途径
- Notch Signaling
抗原
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