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NOTCH4 抗体 (AA 1778-2003)

This 山羊多克隆 anti-NOTCH4 antibody specifically detects NOTCH4 in WB, ELISA 和 ICC. The antibody is reactive with 人 samples.

产品编号 ABIN291848

发货至: 中国

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Quick Overview for NOTCH4 抗体 (AA 1778-2003) (ABIN291848)

抗原

See all NOTCH4 抗体

NOTCH4 (Notch 4 (NOTCH4))

适用

55
39
20
4
4
4
4
3
1
1

人

宿主

山羊

克隆类型

多克隆

标记

37
4
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

This NOTCH4 antibody is un-conjugated

应用范围

39
20
13
13
12
10
7
5
4
3
2
2

Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Discover our top product NOTCH4 Primary Antibody

抗原表位
15

9

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1778-2003

特异性

Recognizes human Notch4.

纯化方法

Affinity purified

免疫原

E. coli-derived rhNotch-4 (aa1778-2003).

亚型

IgG
anti-Notch 4 (NOTCH4) (AA 1715-2001) antibody
NOTCH4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (Middle Region) antibody
NOTCH4 适用: 人, 大鼠, Cow, 马, 豚鼠, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) antibody
NOTCH4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (AA 371-420) antibody
NOTCH4 适用: 人, Cow, 马, 豚鼠, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (AA 1824-2003) antibody
NOTCH4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (AA 1261-1273) antibody
Verified NOTCH4 适用: 人, 小鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (Middle Region) antibody
NOTCH4 适用: 人, Cow, 马, 豚鼠, Pig, 犬, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (C-Term), (cleaved) antibody
NOTCH4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Notch 4 (NOTCH4) (AA 1819-1851), (C-Term) antibody
NOTCH4 适用: 人 WB 宿主: 兔 Polyclonal RB52895 unconjugated

anti-Notch 4 (NOTCH4) antibody
NOTCH4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.

说明

Target Species of Antibody: Human

限制

仅限研究用
状态

Lyophilized

溶解方式

deionized water

浓度

Lot specific

缓冲液

Lyophilized.
抗原

NOTCH4 (Notch 4 (NOTCH4))

别名

NOTCH4

背景

Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

基因ID

4855

UniProt

Q99466

途径

Notch Signaling

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