Interleukin 17a 抗体
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- 抗原 See all Interleukin 17a (IL17A) 抗体
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Interleukin 17a antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Neutralization (Neut), Immunocytochemistry (ICC)
- 特异性
- Recognizes human IL-17.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Purified, E. coli-derived, recombinant human interleukin 17.
Type of Immunogen: Recombinant protein - 亚型
- IgG
- Top Product
- Discover our top product IL17A Primary Antibody
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anti-Interleukin 17A (IL17A) (AA 1-75) antibody
IL17A 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4K5F6 unconjugated
anti-Interleukin 17A (IL17A) (Internal Region) antibodyIL17A 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17A (IL17A) antibodyIL17A 适用: 人 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 17A (IL17A) antibodyIL17A 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17A (IL17A) (AA 24-155) antibodyIL17A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17A (IL17A) (AA 24-133) antibodyIL17A 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 3G5 unconjugated
anti-Interleukin 17A (IL17A) antibodyIL17A 适用: 人 ELISA, FACS, ICC, IP 宿主: 小鼠 Monoclonal 9F9 unconjugated
anti-Interleukin 17A (IL17A) antibody (FITC)IL17A 适用: 人 FACS 宿主: 小鼠 Monoclonal 9F9 FITC
anti-Interleukin 17A (IL17A) antibody (APC)IL17A 适用: 人 FACS 宿主: 小鼠 Monoclonal 9F9 APC
anti-Interleukin 17A (IL17A) antibody (PE)IL17A 适用: 人 FACS 宿主: 小鼠 Monoclonal 9F9 PE
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo, ICC (2 - 15 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA. Neutralization: The ND50 is ~0.1-0.3 μg/mL in the presence of 25 ng/mL of rhIL-17, using the NHDF cell line. Immunocytochemistry: 2-15 μg/mL. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 0.2 ng/well. In this format, this antibody shows approximately 10 % cross-reactivity with rmIL-17 and rhIL-17F and less than 1 % cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D and rhIL-17E. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 5 ng/lane under both non-reducing and reducing conditions. Flow Cytometry: Tested in PBMCs. For intracellular staining to detect IL-17, cells must be first fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled polyclonal antibodies can be visualized by a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.5 mL sterile PBS
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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