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Vitronectin 抗体

This anti-Vitronectin antibody is a 小鼠 单克隆 antibody detecting Vitronectin in WB, ELISA, IHC 和 IHC (p). Suitable for 人, Cow, 犬, 山羊, 马 和 Cat.
产品编号 ABIN2887824
发货至: 中国

Quick Overview for Vitronectin 抗体 (ABIN2887824)

抗原

See all Vitronectin (VTN) 抗体
Vitronectin (VTN)

适用

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人, Cow, 犬, 山羊, 马, Cat

宿主

  • 108
  • 25
  • 4
  • 1
  • 1
小鼠

克隆类型

  • 105
  • 34
单克隆

标记

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This Vitronectin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 品牌

    IHC-plus™

    特异性

    Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

    纯化方法

    Affinity purified

    免疫原

    Human vitronectin purified from plasma by heparin-affinity chromatography.

    Type of Immunogen: Purified protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA (1:30000), IHC, IHC-P (2.5 μg/mL), WB (1:50)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Vitronectin (VTN)

    别名

    VTN / Vitronectin

    背景

    Name/Gene ID: VTN
    Family: Matrix Protein

    Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

    基因ID

    7448

    UniProt

    P04004

    途径

    Autophagy, Smooth Muscle Cell Migration
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