Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
纯化方法
Affinity chromatography purified
免疫原
C-myc antibody (HRP) was raised in goat using a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH as the immunogen.
ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
Supplied as a liquid in 50 mM Hepes buffer, pH 7.1, with 0.0 NaCl, with 0.2 % BSA, and 0.01 % thimerasol.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.