Phosphotyrosine 抗体
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北京 101111
Quick Overview for Phosphotyrosine 抗体 (ABIN285915)
抗原
宿主
克隆类型
标记
应用范围
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交叉反应 (详细)
- Recognize proteins and peptides phosphorylated on tyrosine residues. Does not cross - react with phosphoserine and threonine.
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纯化方法
- Affinity purified
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免疫原
- Phosphotyrosine antibody was raised in rabbit using Iodoacetylphosphotyrosine-KLH conjugate as the immunogen.
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应用备注
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ELISA: 1:1,000, IHC: 1:50, IP: 10 µg/500 µg protein sample, WB: 1:250
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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浓度
- Lot specific
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缓冲液
- Tris-acetate buffer, pH 7-7.5, with 0.05 % NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- -20 °C
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储存方法
- Aliquot and store at -20 °C.
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phosphotyrosine which is considered as a key step in the regulation of enzymatic activities such as signal transduction.
抗原
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