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GAPDH 抗体

Name: GAPDH 抗体
Brand: antibodies-online
SKU: ABIN2857072
Availability: OutOfStock

This anti-GAPDH antibody is a 兔多克隆 antibody detecting GAPDH in WB, IF, IP, ICC 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 105+ publications.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis. (GAPDH 抗体)

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in mouse testis by immunohistochemical analysis. (GAPDH 抗体)

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH, antibody (green) at 1:500 dilution. (GAPDH 抗体)

IP Image Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody, Western blot analysis was performed using GAPDH antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (GAPDH 抗体)

IHC-P Image Immunohistochemical analysis of human salivary gland cancer, using GAPDH, antibody at 1:500 dilution. (GAPDH 抗体)

IHC

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in rat heart by immunohistochemical analysis. (GAPDH 抗体)

IHC

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts. (GAPDH 抗体)

Effects of quercetin on the inflammatory response in H2O2-treated H9C2 cells. (GAPDH 抗体)

Effects of quercetin on cell apoptosis in H2O2-treated H9C2 cells. (GAPDH 抗体)

Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS. (GAPDH 抗体)

Gene trap screen for mitochondrial phenotypes identifies Sucla2 mutant allele. (GAPDH 抗体)

Sucla2 mutant embryos display growth deficiency and placental mtDNA depletion. (GAPDH 抗体)

Sucla2-deficient embryo tissues exhibit progressive, functionally significant mtDNA depletion and elevated MMA. (GAPDH 抗体)

CM extract induces cell apoptosis. (GAPDH 抗体)

Effects of quercetin on doxorubicin-induced changes of cell viability, cell apoptosis and cell morphology in H9C2 cells. (GAPDH 抗体)

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin. (GAPDH 抗体)

RAGE expression in human peripheral nerve fibers. (GAPDH 抗体)

Arecoline induces the transdifferentiation of buccal mucosal fibroblasts (BMFs) into myofibroblasts. (GAPDH 抗体)

Arecoline-induced α-smooth muscle actin (α-SMA) expression is mediated by the direct binding of zinc finger E-box binding homeobox 1 (ZEB1) to the α-SMA promoter. (GAPDH 抗体)

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction. (GAPDH 抗体)

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R). (GAPDH 抗体)

Regulation of NED by REST in LNCaP cells. (GAPDH 抗体)

IL-6 induces NED in LNCaP cells and this is concomitant with increased autophagy. (GAPDH 抗体)

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions. (GAPDH 抗体)

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions. (GAPDH 抗体)

IL-6 treatment inhibits mTOR via the activation of AMPK pathway. (GAPDH 抗体)

Modulation of curcumin in oxidative stress-regulating genes. (GAPDH 抗体)

Arecoline decreased membrane expression of ZO-1 in confluent TM4 cells. (GAPDH 抗体)

Arecoline activates ERK1/2 MAPKs and increases TNF-alpha production. (GAPDH 抗体)

Activation of ERK1/2 MAPKs is important for TNF-alpha induction and ZO-1 protein redistribution by arecoline in TM4 cells. (GAPDH 抗体)

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway. (GAPDH 抗体)

Early and accelerated processing of caspase-8 with DZNep treatment in lymphoma cell lines. (GAPDH 抗体)

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels. (GAPDH 抗体)

Effects of different proteins on post-treatment lipolysis markers. (GAPDH 抗体)

Creation and characterization of Supt4h knockout mice. (GAPDH 抗体)

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A. (GAPDH 抗体)

Dicer inactivation in mutant mice. (GAPDH 抗体)

The expression of BH3-only protein, pro-apoptotic proteins and anti-apoptotic proteins in LNCaP or PC3 cells after treatment with paclitaxel. (GAPDH 抗体)

The effect of ABT-199, ABT-263 or ABT-263 in combination with paclitaxel on LNCaP or PC3 cells. (GAPDH 抗体)

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP. (GAPDH 抗体)

Diverse fuels promote TNKS autoPARsylation and turnover in INS-1 cells. (GAPDH 抗体)

Promoter hypermethylation of sGCβ1 in MCF-7 and sGCα1 in MDA-MB-231 cells. (GAPDH 抗体)

Over-expression of sGCα1 and sGCβ1 in MDA-MB-231 cells affected cGMP production, proliferation, survival, and cell cycle. (GAPDH 抗体)

Over-expression of sGC induced apoptosis and cell cycle arrest. (GAPDH 抗体)

HYS-32 modulates GSK3β phosphorylation. (GAPDH 抗体)

GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes. (GAPDH 抗体)

Caspase-dependent apoptotic pathways were seen in LNCaP, but not in PC3 cells, in response to G2/M arrest. (GAPDH 抗体)

LY294002 inhibits the HYS-32-induced phosphorylation of GSK3β-pS9 and GSK3β-pY216. (GAPDH 抗体)

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL. (GAPDH 抗体)

Effects of TLCA on HDAC4 expression in cultured primary HSCs. (GAPDH 抗体)

Overexpression of miR-29a decreased HDAC4 expression in primary HSCs. (GAPDH 抗体)

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs. (GAPDH 抗体)

Western blot analysis shows pErk reduction through selumetinib treatment and by removal of stromal support. (GAPDH 抗体)

Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation. (GAPDH 抗体)

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation. (GAPDH 抗体)

Mek pathway inhibition in combination-treated BCP-ALL cells by phospho-flow and Western blot. (GAPDH 抗体)

Demyelination/remyelination and decreased P0 expression in dKO mice. (GAPDH 抗体)

Loss of neurofascins and Caspr in dKO mice. (GAPDH 抗体)

P0 interacts with neurofascins. (GAPDH 抗体)

ETO inhibits ACT cell growth. (GAPDH 抗体)

Inhibition of ERK1/2 inhibits ETO-induced centrosome amplification. (GAPDH 抗体)

Chloroquine inhibits DNA damage-induced centrosome amplification. (GAPDH 抗体)

Chloroquine inhibits ETO-activated CDK2 and ERK1/2 in adrenocortical Y1 tumor cells. (GAPDH 抗体)

HSPs positively regulate IE2 transaction activity. (GAPDH 抗体)

Comparison of the mRNA levels of Smad7 (a) and protein levels of Smad3 (b) expressions in the liver of WT and miR-29Tg mice following bile duct ligation (BDL). (GAPDH 抗体)

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation. (GAPDH 抗体)

SFN suppressed HCV protein expression and RNA replication. (GAPDH 抗体)

SFN-mediated HO-1 induction in HCV replicon cells. (GAPDH 抗体)

SFN inhibited HCV replication through HO-1 dependent pathway. (GAPDH 抗体)

SFN induced bilirubin production in HCV replicon cells. (GAPDH 抗体)

SFN inhibited HCV replication by upregulating Nrf2 expression. (GAPDH 抗体)

SFN-mediated Nrf2/HO-1 activation was PI3K dependent. (GAPDH 抗体)

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days. (GAPDH 抗体)

Inhibition of neurite elongation by REST overexpression(A) LNCaP-TR-REST cells were treated with 0. (GAPDH 抗体)

AMPK/mTOR pathway is activated by hypoxia treatment and REST knockdown(A) LNCaP cells were treated with normoxia (N) or hypoxia (H) (2 % O2) for 3 days (left panel). (GAPDH 抗体)

Hypoxia-induced autophagy activation of LNCaP cells(A) LNCaP-eGFP-LC3 cells were treated with hypoxia (2 % O2) for 3 days. (GAPDH 抗体)

Chemical inhibition of autophagy flux by chloroquine (CQ) suppresses hypoxia-induced NED in LNCaP cells(A) LNCaP cells were treated with hypoxia (2 % O2) in the absence or presence of 50 μM CQ for 3 days. (GAPDH 抗体)

Knockdown of beclin 1 suppresses hypoxia-induced NED(A) LNCaP-TR-shBeclin1 cells were treated with 1 μg/mL Dox to induce beclin 1 knockdown for 4 days under normoxia and hypoxia (2 % O2). (GAPDH 抗体)

Disruption of autophagy by treatment with CiQ derivativesA. (GAPDH 抗体)

Induction of apoptosis by treatment with CiQ derivativesA. (GAPDH 抗体)

Induction of lysosome dysfunction by treatment with CiQ derivativesA and B. (GAPDH 抗体)

Differential expression of CDK1 pTyr15 in colorectal cancer cells and tissues. (GAPDH 抗体)

Senataxin is SUMOylated at the XY chromosomes in spermatocytes. (GAPDH 抗体)

120 images

(105 references)

产品编号 ABIN2857072

发货至: 中国

Quick Overview for GAPDH 抗体 (ABIN2857072)

抗原

See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

人

宿主

兔

克隆类型

多克隆

标记

This GAPDH antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

Bacteria, C. elegans, Chanos chanos, 小鸡, Cow, 犬, Drosophila melanogaster, E. coli, 鱼, Hamster, 人, Insect, 猴, Mosquito, 小鼠, Nematode, Ochotona princeps, Pig, 植物, 兔, 大鼠, Saccharomyces cerevisiae, 斑马鱼

产品特性

Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
GAPDH antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

亚型

IgG
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

0.11 mg/mL

缓冲液

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Zhang, Dai, Zhao, Wang, Dou, Zhuang, Chen, Zhao et al.: "MiR-874-3p represses the migration and invasion yet promotes the apoptosis and cisplatin sensitivity via being sponged by long intergenic non-coding RNA 00922 (LINC00922) and targeting ..." in: Bioengineered, Vol. 13, Issue 3, pp. 7082-7104, (2022) (PubMed).

Nakamura, Sato, Kobayashi, Kaneko, Ito, Soma, Okada, Miyamoto, Oya, Matsumoto, Nakamura, Kanaji, Miyamoto: "Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice." in: Scientific reports, Vol. 10, Issue 1, pp. 12242, (2020) (PubMed).

Liao, Zhou, Zhang: "Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2." in: Molecular medicine reports, Vol. 22, Issue 2, pp. 803-809, (2020) (PubMed).

Xu, Li, Wang, Wu, Wang, Chen, Deng, Zhang, Wu, Wan, Liu, Huang, Hu, Zeng, Zhou: "Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock‑in mouse model." in: International journal of molecular medicine, Vol. 46, Issue 3, pp. 1118-1134, (2020) (PubMed).

Liang, Chang, Sheu, Lin, Chung, Teng, Suk: "The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Liu, Du, Rao, Liu, Qu: "Long non-coding RNA NPBWR1-2 affects the development of ovarian cancer via multiple microRNAs." in: Oncology letters, Vol. 20, Issue 1, pp. 685-692, (2020) (PubMed).

Chang, Kam, Chao, Zhao, Chen, Chung, Li, Wu, Lee: "Berberine Derivatives Suppress Cellular Proliferation and Tumorigenesis In Vitro in Human Non-Small-Cell Lung Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).

Low, Lee, Lee, Wang, Chen, Chien, Li, Chang: "Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Möltgen, Piumatti, Massafra, Metzger, Jaehde, Kalayda: "Cisplatin Protein Binding Partners and Their Relevance for Platinum Drug Sensitivity." in: Cells, Vol. 9, Issue 6, (2020) (PubMed).

Bahraoui, Serrero, Planès: "HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway." in: Scientific reports, Vol. 10, Issue 1, pp. 8177, (2020) (PubMed).

Chen, Wu, Ho, Gung, Tsai, Orekhov, Sobenin, Lin, Yet: "Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration." in: International journal of molecular sciences, Vol. 21, Issue 10, (2020) (PubMed).

Baumann, Hägele, Mochayedi, Drebant, Vent, Blobner, Noll, Nickel, Schumacher, Boos, Daniel, Wendler, Volkmar, Strobel, Offringa: "Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy." in: Nature communications, Vol. 11, Issue 1, pp. 2176, (2020) (PubMed).

Yang, Hsieh, Du, Yen, Hung: "Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent Manner." in: Biomolecules, Vol. 10, Issue 4, (2020) (PubMed).

Wang, Yang, Liu, Chun-Kit Tong, Zhu, Malampati, Gopalkrishnashetty Sreenivasmurthy, Cheung, Iyaswamy, Su, Lu, Song, Li: "A Curcumin Derivative Activates TFEB and Protects Against Parkinsonian Neurotoxicity inVitro." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Liu, Chen, Lii, Jhuang, Huang, Li, Yao: "A Diterpenoid, 14-Deoxy-11, 12-Didehydroandrographolide, in Andrographis paniculata Reduces Steatohepatitis and Liver Injury in Mice Fed a High-Fat and High-Cholesterol Diet." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Loh, Tsai, Huang, Yu, Kuo, Chao, Chou, Tsai, Lee: "Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †." in: Cancers, Vol. 12, Issue 2, (2020) (PubMed).

Yang, Zhu, Tong, Iyaswamy, Xie, Zhu, Sreenivasmurthy, Senthilkumar, Cheung, Song, Zhang, Li: "A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38." in: Redox biology, Vol. 32, pp. 101445, (2020) (PubMed).

Chang, Chu, Tsai, Lai, Chen: "Dopamine D3 receptor and GSK3β signaling mediate deficits in novel object recognition memory within dopamine transporter knockdown mice." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 16, (2020) (PubMed).

Wang, Wang, Lin, Kao, Hung, Teng, Tsai, Chi: "Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis." in: Aging cell, Vol. 19, Issue 2, pp. e13090, (2020) (PubMed).

Huang, Chyuan, Shiue, Yu, Hsu, Hsu: "Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1822-1836, (2020) (PubMed).

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抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

glyceraldehyde-3-phosphate dehydrogenase

背景

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Cellular Localization: Cytoplasm , perinuclear region , Membrane

分子量

36 kDa

基因ID

2597

UniProt

P04406