电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

S1PR1 抗体 (C-Term)

This anti-S1PR1 antibody (ABIN2857018) is a Rabbit Polyclonal antibody detecting S1PR1 in WB, IF, IHC (p), ICC. Suitable for Human.
产品编号 ABIN2857018
发货至: 中国

Quick Overview for S1PR1 抗体 (C-Term) (ABIN2857018)

抗原

See all S1PR1 抗体
S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

适用

  • 99
  • 65
  • 43
  • 7
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1

宿主

  • 97
  • 11

克隆类型

  • 86
  • 22
多克隆

标记

  • 61
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This S1PR1 antibody is un-conjugated

应用范围

  • 79
  • 41
  • 18
  • 17
  • 14
  • 13
  • 13
  • 11
  • 7
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • 抗原表位

    • 15
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    交叉反应

    人, 小鼠

    产品特性

    Rabbit Polyclonal antibody to EDG1 (sphingosine-1-phosphate receptor 1)
    EDG1 antibody [C2C3], C-term

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: HepG2

    限制

    仅限研究用
  • 生效 #100014 (Immunofluorescence)
    '独立验证'标志
    by
    Memorial Sloan-Kettering Cancer Center, Parada lab
    No.
    #100014
    日期
    2016.05.11
    抗原
    Sphingosine-1-Phosphate Receptor 1 (S1PR1)
    Lot Number
    39471
    Method validated
    Immunofluorescence
    Positive Control
    Wild-type mouse adult brain cryo-sections
    Negative Control
    Rabbit IgG
    Notes
    The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2857018
    Secondary Antibody
    Donkey-anti-Rabbit-Cy3
    Full Protocol
    • Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
    • Immunostaining for Cryo Sections:
      • Dry slides at 50-55°C for 15-30min (or RT for 30min).
      • Wash with PBS 5min X 3 (shake gently).
      • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
      • Incubate with ABIN2857018 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
      • Wash with PBS 5min X 3.
      • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
      • Wash with PBS 5min X 3.
      • Add three drops of water-based Immu-mount.
      • Cover with 24 X 60 cover glass.
    • Imaging:
      • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
    Experimental Notes
    ABIN2857018 worked the best at 1:200. At 1:400, or 1:800, it seems to lose some signals in some cells.
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

    别名

    sphingosine-1-phosphate receptor 1

    背景

    The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.

    Cellular Localization: Cell membrane, Multi-pass membrane protein

    分子量

    43 kDa

    基因ID

    1901

    UniProt

    P21453

    途径

    Signaling Events mediated by VEGFR1 and VEGFR2
You are here:
Chat with us!