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DENND1B 抗体 (Center)

This anti-DENND1B antibody is a 兔 多克隆 antibody detecting DENND1B in WB, ICC 和 IF. Suitable for 人 和 小鼠.
产品编号 ABIN2856495
发货至: 中国

Quick Overview for DENND1B 抗体 (Center) (ABIN2856495)

抗原

See all DENND1B 抗体
DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This DENND1B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • 抗原表位

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    Center

    产品特性

    Rabbit Polyclonal antibody to DENND1B (DENN/MADD domain containing 1B)
    DENND1B antibody [N1C1]

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human DENND1B. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

    说明

    Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.9 mg/mL

    缓冲液

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

    别名

    DENND1B

    背景

    Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM]

    分子量

    48 kDa

    基因ID

    163486
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