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PD-L1 抗体 (C-Term)

Name: PD-L1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2855940
Availability: OutOfStock

This anti-PD-L1 antibody is a 兔多克隆 antibody detecting PD-L1 in WB, FACS, IF, ICC, IHC (p) 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 7+ publications.

WB Image A549 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody , diluted at 1:500. (PD-L1 抗体 (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PD-L1, antibody at 1:100 dilution. (PD-L1 抗体 (C-Term))

IHC

WB Image Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody , diluted at 1:500. (PD-L1 抗体 (C-Term))

3 images

(7 references)

产品编号 ABIN2855940

发货至: 中国

Datasheet as PDF

Quick Overview for PD-L1 抗体 (C-Term) (ABIN2855940)

抗原

See all PD-L1 抗体

PD-L1 (CD274 (PD-L1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PD-L1 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

质量等级

KO Validated

Discover our top product PD-L1 Primary Antibody

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C-Term

交叉反应

人

产品特性

Rabbit Polyclonal antibody to PD-L1 (CD274 Molecule)
PD-L1 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

亚型

IgG
anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated

anti-CD274 (PD-L1) (AA 39-191) antibody
PD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated

anti-CD274 (PD-L1) (AA 48-290) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 34-241) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated

anti-CD274 (PD-L1) (AA 201-290) antibody
PD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-234) antibody
PD-L1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A431(100 ng/mL IFN-gamma treatment for 48 hr)
Validation: Comparison, KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.29 mg/mL

缓冲液

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Kim, Kim, Kim, Kim, Kim: "Differential Expression and Clinicopathological Significance of HER2, Indoleamine 2,3-Dioxygenase and PD-L1 in Urothelial Carcinoma of the Bladder." in: Journal of clinical medicine, Vol. 9, Issue 5, (2020) (PubMed).

Qin, Wang, Ye, Li, Chen, Wang, Qin, Zhang, Li, Long, Hu, Shi, Li, Zhang, Zhai, Tang, Kang, Lan, Xie, Lu, Deng: "NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer." in: Nature communications, Vol. 11, Issue 1, pp. 1669, (2020) (PubMed).

Gondhowiardjo, Handoko, Adham, Rachmadi, Kodrat, Tobing, Haryoga, Dwiyono, Kristian, Mayang Permata: "Tumor microenvironment predicts local tumor extensiveness in PD-L1 positive nasopharyngeal cancer." in: PLoS ONE, Vol. 15, Issue 3, pp. e0230449, (2020) (PubMed).

Pan, Wu, Kan, Li, Chang, Wu, Li, Ou-Yang, Hou, Yip, Luo: "Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer." in: Cancers, Vol. 12, Issue 1, (2019) (PubMed).

Cha, Yang, Xia, Wei, Chan, Lim, Li, Kim, Chang, Lee, Hsu, Wang, Kuo, Chang, Hadad, Purdie, McCoy, Cai, Tu, Litton, Mittendorf, Moulder, Symmans, Thompson, Piwnica-Worms, Chen, Khoo, Hung: "Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1." in: Molecular cell, Vol. 71, Issue 4, pp. 606-620.e7, (2019) (PubMed).

Buisseret, Garaud, de Wind, Van den Eynden, Boisson, Solinas, Gu-Trantien, Naveaux, Lodewyckx, Duvillier, Craciun, Veys, Larsimont, Piccart-Gebhart, Stagg, Sotiriou, Willard-Gallo: "Tumor-infiltrating lymphocyte composition, organization and PD-1/ PD-L1 expression are linked in breast cancer." in: Oncoimmunology, Vol. 6, Issue 1, pp. e1257452, (2017) (PubMed).

Lin, Sung, Hsieh, Tsai, Lai, Yang, Shen, Chen, Lee, Yeh, Chen: "High PD-L1 Expression Correlates with Metastasis and Poor Prognosis in Oral Squamous Cell Carcinoma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142656, (2016) (PubMed).
抗原

PD-L1 (CD274 (PD-L1))

别名

CD274 molecule

背景

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Cellular Localization: Isoform 1: Cell membrane, Single-pass type I membrane protein , Isoform 2: Endomembrane system, Single-pass type I membrane protein

分子量

33 kDa

基因ID

29126

UniProt

Q9NZQ7

途径

Cancer Immune Checkpoints