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RFC4 抗体

This anti-RFC4 antibody is a 兔 多克隆 antibody detecting RFC4 in WB, IF, IHC (p) 和 ICC. Suitable for 人.
产品编号 ABIN2855757
发货至: 中国

Quick Overview for RFC4 抗体 (ABIN2855757)

抗原

See all RFC4 抗体
RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

适用

  • 45
  • 20
  • 15
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1

宿主

  • 35
  • 8
  • 4

克隆类型

  • 41
  • 6
多克隆

标记

  • 36
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RFC4 antibody is un-conjugated

应用范围

  • 36
  • 12
  • 12
  • 10
  • 6
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • 交叉反应

    人, 小鼠

    产品特性

    Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37 kDa)
    RFC4 antibody [N1C3]

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.62 mg/mL

    缓冲液

    0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

    别名

    replication factor C subunit 4

    背景

    The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

    Cellular Localization: Nucleus

    分子量

    40 kDa

    基因ID

    5984

    UniProt

    P35249

    途径

    Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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