RFC4 抗体
Quick Overview for RFC4 抗体 (ABIN2855757)
抗原
See all RFC4 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 人, 小鼠
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产品特性
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Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37 kDa)
RFC4 antibody [N1C3] -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.
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亚型
- IgG
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anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 254-363) antibody
RFC4 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 1C12 unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 204-363) antibodyRFC4 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 1-363) antibodyRFC4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) antibodyRFC4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 1-363) antibodyRFC4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 9-142) antibodyRFC4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) antibodyRFC4 适用: 人, 小鼠, 大鼠 WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 4, 37kDa (RFC4) (AA 287-336) antibodyRFC4 适用: 人, 小鼠, 大鼠, 犬, 马, Bat, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.62 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))
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别名
- replication factor C subunit 4
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背景
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The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Cellular Localization: Nucleus -
分子量
- 40 kDa
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基因ID
- 5984
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UniProt
- P35249
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
-
