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PRKAA2 抗体 (Center)

PRKAA2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2855639
发货至: 中国
  • 抗原 See all PRKAA2 抗体
    PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
    抗原表位
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    Center
    适用
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    人, 小鼠
    宿主
    • 115
    • 12
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    • 1
    克隆类型
    • 108
    • 23
    多克隆
    标记
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    This PRKAA2 antibody is un-conjugated
    应用范围
    • 116
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    交叉反应
    小鸡, 猪, 大鼠, 牛, Xenopus tropicalis
    交叉反应 (详细)
    Chicken (96 %), Pig (99 %), Rat (98 %), Bovine (99 %), Xenopus tropicalis (96 %)
    产品特性
    Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)
    AMPK alpha 2 antibody
    纯化方法
    Purified by antigen-affinity chromatography.
    免疫原
    Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
    亚型
    IgG
  • 应用备注
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
    说明

    Positive Control: A431 , HeLa , A375 , NIH-3T3

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.55 mg/mL
    缓冲液
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Gao, Yuan, Wang, Lei, Shen, Cui, Zhang, Ding, Lu: "Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." in: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).

    Zhang, Zhang, Zhou, Fu, Michal, Ji, Du, Davis, Jiang: "Alternative polyadenylation drives genome-to-phenome information detours in the AMPKα1 and AMPKα2 knockout mice." in: Scientific reports, Vol. 8, Issue 1, pp. 6462, (2018) (PubMed).

    Lin, Kiihl, Suhail, Liu, Chou, Kuang, Lu, Khor, Lin, Bader, Irizarry, Boeke: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).

  • 抗原
    PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
    别名
    AMPK alpha 2 (PRKAA2 产品)
    别名
    AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, AMPKA2 antibody, ampk antibody, ampk2 antibody, prkaa antibody, prkaa1 antibody, PRKAA2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antibody, PRKAA2 antibody, Prkaa2 antibody, prkaa2.S antibody, prkaa2 antibody
    背景
    The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

    分子量
    62 kDa
    基因ID
    5563
    途径
    AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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