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MDC1 抗体

Name: MDC1 抗体
Brand: antibodies-online
SKU: ABIN2855454
Availability: OutOfStock

This anti-MDC1 antibody is a 兔多克隆 antibody detecting MDC1 in WB, IF, IHC (p) 和 ICC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1, antibody at 1:500 dilution. (MDC1 抗体)

IHC

WB Image Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE antibody diluted at 1:1000 (MDC1 抗体)

SMYD3 knockdown downregulates HR gene expressions. (MDC1 抗体)

3 images

(2 references)

产品编号 ABIN2855454

发货至: 中国

Quick Overview for MDC1 抗体 (ABIN2855454)

抗原

See all MDC1 抗体

MDC1 (Mdc1 (MDC1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This MDC1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Discover our top product MDC1 Primary Antibody

交叉反应

人

产品特性

Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)
MDC1 antibody [N2N3]

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.

亚型

IgG
anti-Mdc1 (MDC1) (AA 1-100) antibody
MDC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mdc1 (MDC1) (AA 31-130) antibody
MDC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mdc1 (MDC1) (AA 1-350) antibody
MDC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mdc1 (MDC1) (N-Term) antibody
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 unconjugated

anti-Mdc1 (MDC1) (N-Term) antibody (HRP)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 HRP

anti-Mdc1 (MDC1) (N-Term) antibody (FITC)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 FITC

anti-Mdc1 (MDC1) (N-Term) antibody (Biotin)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 Biotin

anti-Mdc1 (MDC1) (N-Term) antibody (PE)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 PE

anti-Mdc1 (MDC1) (N-Term) antibody (APC)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 APC

anti-Mdc1 (MDC1) (N-Term) antibody (PerCP)
MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 PerCP

应用备注

WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HeLa nucleus
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Burger, Schlackow, Gullerova: "Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." in: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).

Chen, Tsai, Wang, Teng: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." in: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).
抗原

MDC1 (Mdc1 (MDC1))

别名

mediator of DNA damage checkpoint 1

背景

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Cellular Localization: Nucleus

分子量

227 kDa

基因ID

9656

UniProt

Q14676